Composite

Part:BBa_K2525018:Design

Designed by: Kshitij Rai   Group: iGEM17_IIT_Delhi   (2017-10-27)


RBS + SrpR + T


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 509
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since the part was Biobrick RFC10 compatible, we did not face any significant issues with its design or usage.


Source

The part comes from the genomic sequence of Pseudomonas putida, and was obtained as part of a collaboration with iGEM Glasgow

References